Application: Aequorin-Based Calcium Assay
- Determination of intracellular calcium
- Detection of for calcium sensitive Aequorin
Fluorescent Proteins
Aequorin was originally obtained by the purification from the jellyfish (Aequorea victoria), but now it is prepared more efficiently using recombinant techniques. The active aequorin is formed by a complex between apoaequuorin (APO), oxygen and coelenterazine. On binding calcium, the complex is broken emitting light and rendering the free apoaequorin and coeleteramide.
Active aequorin can be directly microinjected or obtained by reconstitution of apoaequorin with coelenterazine in transfected cells. In both cases, coelenterazine is needed to obtain the active complex (coelenterazine is burn out after light emission and is needed for reconstitution of aequorin). When using cells transfected with apoaequorin cDNA, the rate-limiting step in the reconstitution is the cell permeation of coelenterazine. Reconstitution of aequorin can be done using the native coelenterazine or analogs (some analogs with quicker response and more intese signal have been described and are comercially available). Although this protein was very popular for quantifying calcium in the past 30 years, it has been substituted by GFP derivatives. New derivatives combining aequorin and GFP or RFP (red fluorescence protein) have been developed based on the chemiluminescence resonance energy transfer process (CRET or BRET)¹.
White pigmented plates are optimized for luminescence measurements. Because of the white coloration, the plates show a maximum reflection of the luminescence signals and minimize well-to-well crosstalk. White plates are available in standard or with transparent bottom. The white pigmented plates are available in the 96-, 384-, and 1536-well standard format and with transparent bottom. The microplates are offered in non-treated, immunological, and cell culture surfaces.
Application Criteria
- Surface Type: Non-Treated, Immunoassay, and Cell Culture
- Cavities: 96-well, 384-well, and 1536-well
- Material: Polystyrene
- Well Plate Types: Standard and Transparent Bottom
- Color: White Pigmented Microplates
- Bottom Types: F-Bottom, C-Bottom, and U-Bottom,
Product Selection Table
To help guide you through the microplate selection process we have developed a comprehensive selection table for white pigmented plates below. Products can be sorted by item number, surface type, number of cavities, microplate material, plate type, microplate color, bottom type and volume.
Questions?
This is a comprehensive list of white pigmented microplates, if you have a specific question please feel free to contact us for more information.
Microplate Sample Program
We understand that selecting the right microplate is an important consideration and that is why we are excited to invite you to try our risk free sample program so that you can try the microplate in your laboratory before making your initial purchase.
| Cat. No. | Surface | Cavities | Material | Type | Color | Bottom type | Volume |
|---|---|---|---|---|---|---|---|
| 781604 | pureGrade™ | 96-well | PS | Standard | white | U | 330 µl |
| 781605 | pureGrade™ | 96-well | PS | Standard | white | F | 350 µl |
| 781610 | pureGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781621 | pureGrade™ | 384-well | PS | Standard | white | F | 100 µl |
| 781626 | pureGrade™ | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 781641 | pureGrade™ | 1536-well | PS | Standard | white | F | 10 µl |
| 781665 | pureGrade™ S | 96-well | PS | Standard | white | F | 350 µl |
| 781670 | pureGrade™ S | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781681 | pureGrade™ S | 384-well | PS | Standard | white | F | 100 µl |
| 781686 | pureGrade™ S | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 781701 | pureGrade™ S | 1536-well | PS | Standard | white | F | 10 µl |
| 781724 | immunoGrade™ | 96-well | PS | Standard | white | U | 330 µl |
| 781726 | immunoGrade™ | 96-well | PS | Standard | white | C | 350 µl |
| 781741 | immunoGrade™ | 384-well | PS | Standard | white | F | 100 µl |
| 781784 | hydroGrade™ | 96-well | PS | Standard | white | U | 330 µl |
| 781786 | hydroGrade™ | 96-well | PS | Standard | white | C | 350 µl |
| 781801 | hydroGrade™ | 384-well | PS | Standard | white | F | 100 µl |
| 781844 | lipoGrade™ | 96-well | PS | Standard | white | U | 330 µl |
| 781846 | lipoGrade™ | 96-well | PS | Standard | white | C | 350 µl |
| 781850 | lipoGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781852 | lipoGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781861 | lipoGrade™ | 384-well | PS | Standard | white | F | 100 µl |
| 781866 | lipoGrade™ | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 781868 | lipoGrade™ | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 781904 | inertGrade™ | 96-well | PS | Standard | white | U | 330 µl |
| 781905 | inertGrade™ | 96-well | PS | Standard | white | F | 350 µl |
| 781906 | inertGrade™ | 96-well | PS | Standard | white | C | 350 µl |
| 781910 | inertGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781912 | inertGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781965 | cellGrade™ | 96-well | PS | Standard | white | F | 350 µl |
| 781970 | cellGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781974 | cellGrade™ | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 781981 | cellGrade™ | 384-well | PS | Standard | white | F | 100 µl |
| 781986 | cellGrade™ | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 781988 | cellGrade™ | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 782001 | cellGrade™ | 1536-well | PS | Standard | white | F | 10 µl |
| 782025 | cellGrade™ plus | 96-well | PS | Standard | white | F | 350 µl |
| 782030 | cellGrade™ plus | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 782034 | cellGrade™ plus | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 782046 | cellGrade™ plus | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 782048 | cellGrade™ plus | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 782085 | cellGrade™ premium | 96-well | PS | Standard | white | F | 350 µl |
| 782090 | cellGrade™ premium | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 782094 | cellGrade™ premium | 96-well | PS | Transp. Bottom | white | F | 330 µl |
| 782106 | cellGrade™ premium | 384-well | PS | Transp. Bottom | white | F | 120 µl |
| 782108 | cellGrade™ premium | 384-well | PS | Transp. Bottom | white | F | 120 µl |