96-Well Plate Footprint Dimensions
Microplate Standard Overview
The standard established by the former Society for Biomedical Sciences (SBS) now the Society for Laboratory Automation and Screening (SLAS) defines the dimensional requirements of the footprint of a microplate as specified in the American National Standards covering these microplates. This standard was devised to describe the minimal dimensions required of a microplate to meet the standard. The standard serves as a guide for the conditions required for making necessary measurement. According to the SLAS, unless otherwise specified, all dimensions are applicable at 68 degrees Fahrenheit (20 degree Celsius). Applicable compensation may be made for measurements at other temperatures.
Normative References
The following standards contain provisions which, through reference in this text, constitute provisions of this American National Standard. At the time of publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this American National Standard are encouraged to investigate the possibility of applying the most recent editions of the standards listed below¹.
ASME Y14.5M-1994, Dimensioning and Tolerancing
96-Well Standard Microplate Footprint
4 Microplate footprint
4.1 Normal Tolerances
4.1.1 Footprint
4.1.1.1
The outside dimension of the base footprint, measured within 1.7 mm (0.5000 inches) of the outside corners, shall be as follows:
x Length 127.76 mm ± 0.5 mm (5.0299 inches ± 0.0197 inches)
x Width 85.48 mm ± o.5 mm (3.3654 inches ± 0.0098 inches)
4.1.1.2
The outside dimension of the base footprint, measured at any point along the side, shall be as
follows:
x Length 127.76 mm ± 0.5 mm (5.0299 inches ± 0.0197 inches)
x Width 85.48 mm ± 0.5 mm (3.3654 inches ± 0.0197 inches)
4.1.1.3 The footprint must be continuous and uninterrupted around the base of the plate.
4.1.2 Corner Radius
4.1.2.1
The four outside corners of the plate’s bottom flange shall have a corner radius to the outside of the 3.18 ± 1.6 mm (0.1252 inch ± 0.0630)
Complete Specifications Available
The ANSI/SLAS 1-2004 Footprint Dimension standards are comprehensive, therefore the information contained in this post is an abbreviated version highlighting the overall footprint dimensions. To access the entire PDF document, please visit the Society for Laboratory Automation and Screening website (https://www.slas.org/default/assets/File/ANSI_SLAS_1-2004_FootprintDimensions.pdf)
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